1. The total alkaloids from Zanthoxylum nitidum plays a protective action against gastric ulcer. Its anti-ulcer effect is related to NO increase in serum and gastric mucosa, and PGE₂ content in plasma^[1].

2. The extract from the roots and stems of Zanthoxylum nitidum DC. can show anti-inflammatory and analgesic activities, whereas the activities of the roots were better than the stems^[2].

3. The extract of the roots of Zanthoxylum nitidum(S-0) has significant efficacies on analgesia, anti-inflammation and hemostasia in mice^[3].

4. The extract from Zanthoxylum nitidum (Roxb.) DC. exhibits effect on inhibiting whole blood chemiluminescence and lipid peroxidate in the cells of the liver induced by cysteine Fe²⁺, and could scavenge O^-2 in alkaline/pyrogallos system, which show Zanthoxylum nitidum (Roxb.)DC. may be used as an antioxidant^[4].

5. The water extract of Zanthoxylum nitidum DC could effectively reduce the level of ALT, AST and MDA, increase the activity of SOD, and has a protective effect on acute hepatic injury mice induced by CCl₄^[5].

6. Seven alkaloids were isolated from the roots of Zanthoxylum nitidum (Roxb.) DC and six of them were identified as nitidine chloride (1), oxynitidine (2), dihydronitidine (3), 6-methoxy-5, 6-dihydrochelerythrine (4), α-allocryptopine (5) and skimmianine (6). The structural study on 7 is in progress.1 and 4 were able to increase the life span of mice inoculated with Ehrlieh’s ascites tumor, while 2, 3, 5 and 6 were inactive. 1 was effective in the treatment of chronic myelocytic leukemia^[6].

7. Nitidine chloride has significant antitumor effect in vivo, and its influence on oncogene may be one of its anti-tumor mechanism^[7].

Liangmianzhen(Zanthoxylum nitidum)

1.Dentine Hypersensitivity: The total alkaloids extract of liangmianzhen (Zanthoxylumnitidumextract) (extracted with alcohol). Checking the sensitiveregion with a probe, it divided into mild, moderate and severe sensitiveaccording to Crossman’s grading standard. After flushing and insulate wet theSick teeth. the treatment group used small cotton dip the extract ofliangmianzhen(Zanthoxylum nitidumextract)into the sensitive region, which of the roast hot filling applicator on cottonhead, it is appropriate for the sick cantolerate to the patient. Repeat 2~3 times. Using diathermy once a week, threetimes for a treatment, if symptoms disappear after one treatment, can no longertreatment, if without improving even after three treatment courses, it is invalid,terminate the treatment. Control group was used a small cotton dipping moderateSodium Fluoride into the sensitive region again and again for one to twominutes, once a week, 3 times a treatment. Results: 122 cases for treatmentgroup, desensitization immediately for 118 cases(96.7%), desensitize one monthlater for 111 cases(91.0%), and one year later for 105 cases (86.1%); thecontrol group was 40 cases, desensitization immediately for 33 cases(82.5%), desensitizeone month later for 30 cases(75.0%), one year later for 19 cases (47.5%),compared the control group with the treatment group, the difference wassignificant (P<0.01) ^[1].

2.Every kind of pain: Make the extract of liangmianzhe (Zanthoxylumnitidumextract) into tablets, 2-3 pieces a time. Cured various pain 96cases(Gastrointestinal pain 37 cases), effective 87 cases, invalid 9 cases, thetotal effective rate was 90.6%; toothache patients 14 cases, the totaleffective rate was 92.8%. The best effect to the patients wasn’t guminflammation. But for Cancer pain, Traumatic pain, Postoperative wound pain,Limbs pain was in vain. After taking the drug adverse relations were no found ^[2].      .

1.Dental caries with pain

2.Scald

3.Nameless sore or boil

4.Traumatic Injury

5.Rheumatic arthritis

6.Amenorrhea

7.Biliary ascariasis

8.Intercostal neuralgia

9.Gastric and duodenal ulcer

10.Dysentery

[1] PANG Hui, HE Hui, JIA Xiao-dong, et al. Effect of Total Alkaloids from Zanthoxylum nitidum on Gastric Mucosal Defensive Factors in Rats with Gastric Ulcer. Lishizhen Medicine and Materia Medica Research, 2007,18(3):533-534.

[2] Feng Jie, Zhou Jingfan, Qin Fujing, et al. Studies on the anti-inflammatory and analgesic activities of different polarities from the roots and stems of Zanthoxylum nitidum DC. Pharmacology and Clinics of Chinese Materia Medica, 2011,27(6):60-63.

[3] LIU Shao-hua, QIN Qing-yun, FANG Kun, et al. The Effects of the Extract(S-0) from Zanthoxylum nitidum on Analgesia,Anti-inflammation and Hemostasia in Mice. Natural Product Research and Development, 2005,17(6):758-761.

[4] XIE Yun-feng. Antioxidative Effect of Extracts from Zanthoxylum nitidum (Roxb.) DC. LiShiZhen Medicine and Materia Medica Research, 2000,11(1):1-2.

[5] PANG Fei, TANG Gui-fang, HE Hui, et al. The Protective Effect of Zanthoxylum nitidum DC. on Acute Hepatic Injury Mice Induced by CCl₄. Guangxi Medical Journal, 2006,28(10):1606-1608.

[6] HUANG Zhi-Xun, LI Zhi-he. STUDIES ON THE ANTITUMOR CONSTITUENTS OF ZANTHOXYLUM NITIDUM (ROXB.) DC. Acta Chimica Sinica, 1980,38 (6):535-542.

[7] LIU Li-min, LIU Hua-gang, LUO Dan. Study on the Anti-tumor Effect in vivo of Nitidine Chloride and its Mechanism. Lishizhen Medicine and Materia Medica Research, 2009,20(6):1389-1390.
Clinical experiment

[1] Feng riping.Clinical observation on the treatment of Chinese Herbal medicine Zanthoxylumnitidumextract extract to Dentine Hypersensitivity. GuangDong Prevention andControl of odontia. 2001,9（3）:193.

[2]Chen zhaosen. Clinical observation on the effective of the active constituentof anthoxylum nitidumextract ta pain. Journal of Chinese Herbal medicine.1982,13（5）:240.