1 Cardiovascular Systemeffect: The blood pressure ofanesthetized rabbits was significantly decreased by giving anthraquinone. Theheart rates of the anesthetized rabbits were decreased significantly by givinganthraquinone, except that any other change was not found inelectrocardiograph[1]. PD could reduce the adhesion of neutrophil-EC directly,which may be one ofthe reasons that PD can improve the microcirculation [2]. Polydatin reducedFIB, PAdT in rat model remarkably. It obviously caused a reduction ofηp, whichinduced decrease ofηb. The effect was more significant than any other factorsin reducing blood viscosity and improving microcirculation [3]. PD did notchange the k_ATP channel conductance. However, PD changed the K_ATPchannel kinetics, Tom and Open probability (Po) increased significantly. Theresults suggested that K_ATP channels were activated. Polydatin hasthe effect of activating K_ATP channels of ASMCs, which mightcontribute to arteriolar dilation and microcirculation improvement byhyperpolarizing arteriolar smooth muscle cells. It decrease platelet free-Ca²⁺induced by thrombin without affecting the rate of Ca²⁺ uptake.Polydatin was shown to inhibit ADP, arachidonic acid, Ca²⁺ andclonidine-induced platelet aggregation significantly. In vitro, the rates ofinhibition were 18～36% （ADP-induced）, 26～58% （arachidonic acid-induced） and 42～64% (Ca²⁺-induced). However, the inhibition of thrombin-inducedaggregation was not statistically significant [4-7]. In vivo experiments, 5, 30and 60 min after iv polydatin, the rates of inhibition were 52～63% （ADP-induced）, 59～76% （arachidonicacidinduced） and 10～16% (Ca²⁺-induced) respectively. polydatin caused apersistent and dose-dependent increase of intracellular calciumconcentration[Ca 2+ ]i,which were inhibited partly by pretreatment with calciumchelator EGTA (2 mmol/L) and calcium channel blocker verapamil (50μmol/L)respectively 10 min prior to polydatin administration. polydatin-induced[Ca²⁺] alternation are not only due to extracellular Ca²⁺inflow but also Ca²⁺ release from intracellular calcium store. Polydatin-inducedalternation in [Ca²⁺] might be mainly due to the entry ofextracellular Ca²⁺ into cells and release from IP3 sensitiveintracellular calcium store, and ryanodine receptor system might regulatenegatively IP3 receptor system. And polydatin might induce the entry of sodiumion into cells and cause the depolarization of VSMC. The infusion of polygonumcuspidatum caused a continuous and dramatic reduction of the mean systolicpulmonary arterial pressure (P<0.01) and increase of cardiac index(P<0.05) while the systemic blood pressure, heart rate and indices of bloodgas analysis remained unchanged. The drug also significantly increased theactivity of tissue plasminogen activator (P<0.01) and decreased the activityof plasminogen activator inhibitor (P<0.05) [8-11]. The levels of D dimerand antithrombin Ⅲshowed no changes. It is concluded that Polygonum cuspidatum can ameliorate thehypoxic pulmonary hypertension, increase the cardiac output, and enhance thefibrinolytic activity, but with no effect on the systemic blood pressure andoxygenation of hypoxic pigs. PD enhanced the release of PGI2 and theenhancement was correlated to dosage. 6-keto-PGF1α were 430±220 and 330± 120pg/ml respectively when PD 0.46mmol/L and 0.15mmol/L were separatelyadministered by 10 minutes. The enhancing effect of the doses remainedsignificantly effective 30 minutes after administration. No enhancing responsewere seen using lower than the concentration of 0.15 mmol/L. There were noinfluence of PD on morphologic changes of endothelial cells of umbilical vein.Polydatin presented obvious antithrombotic effects, polydatin has evidentantithrombotic effects in artery, vein and micro-circulation. polydatin caninhibit the platelet aggregation and thrombosis. High-fat-diet causedhyperlipidemia [12-16]. The content of TC, TG, LDL-c and NO and activities ofNOS and iNOS in serum were decreased significantly in the hyperlipidemia ratswith the piceid compared with high lipid control group. In comparison with highlipid control group, activities of serum cNOS increased in the hyperlipidemiarats with the piceid. Moreover, piceid could decrease AI and increase ratio ofHDL-c/TC. Piceid can regulate blood lipid and free radical metabolism inhyperlipidemia rats. 3 Pol groups, cerebra l water content, MDA content, LAcontent and MAO activity were much higher in mo del group(P<0.01). The SODactivity was lower in model group with a signifi cant difference between shamand 3 Pol groups (P<0.01). Conclusion:Polydatin has a great protectiveeffect on cere bral ischemia-reperfusion injury. The activity of PKC ofmyocardial cells cytoplasm in normal situation after the cells were treated byPD decreased(vs normal group P<0.05), but membrane increased (vs normalgroup P<0.05). After myocardial cells were in ischemia and hypoxiasituation, the activity of PKC of cytoplasm and membrane decreased(vs normalgroup P<0.05), but activity of PKC increased again by PD treatment(vsischemia and hypoxia group P<0.05, vs normal group P>0.05). Afterinfusing introvanously polygonum cuspidatum injection,the mean value ofpulmonary artery pressure was significantly decreased than that before therapyin pigs and in controls (P<0.01);Cardiac Index was increased in treatmentgroups than that in controls (P<0.05);The mean value of t-PA was increasedthan that both before therapy in pigs and in controls(P<0.01);The mean valueof PAI was decreased than that both before therapy in pigs and incontrols(P<0.05). The results suggest that polygonum cuspidatum could significantlydecline the pulmonary artery pressure in hypoxic animals and it could increasethe cardiac output and improve the fibrinolitic activity. LPS may directlyinduce stress fiber formation, therefore cause damages to rat cardiac myocytes,which can be reverted by polydatin through the mechanism of participating inthe F-actin organization [17-20].

2 Protectingliver: Polygoum cuspidatum sieb posseses oxidatico resistance and can reducethe intensity of liver function impeirment. The extracts of rizoma polygoni cuspidatican improve the adipose and glucose metabolism of NAFLD rats [21]. It candecrease the contents of triglycerde, total cholesterol, and glucose in liverand serum. PD could protect primary cultured rat hepatocytes against CCl₄induced injury [22].

3 Antibacterial activity：PCWEcan partly inhibit LP BM5 MuLV induced splenomegaly, immunodeficiency andviremia of C57BL/6 mice [24-26]. Antiviral effect of PCWE is more effectivebefore or at the same time of virus inoculation than after virus inoculation[27]. It indicates that the earlier treatment, the better. Continual treatmentcan partly improve splenomegaly and ConA response of infected mice, but theinhibition of PCWE on hypergrmmaglobulinemia of infected mice is non persistent[28-31] .

4 Gastrointestinal effect: The index of experimental gastricmucosal lesion in rats was inhibited after administration of PD, the decreaseof SOD of serum was elevated to normal level [32]. PD can possess protectiveeffect on experimental gastric mucosal lesion in rats.Its mechanism may berelated to antioxidative effect [33].

5 Relieving cough and Antiasthma: both Emodin and PD had relieving cough effects [32].

6 Antitumor: The effect compound (Resveratrol) can inhibitthe growth and proliferation of mouse hepatocellular carcinoma H22 in vitrowhose probable mechanism is induce apoptosis of H22 cells [34].

7 Hemostasis: Polygonihad obvious hemostatic effect on topical traumatic bleeding [28], andhemostatic effect of upper gastrointestinal bleeding.

1. Herpes Zoster:

(1) 88 cases of facial herpes zoster patients were treated by Fufang Huzhang tincture(Huzhang 100g, Guanzhong50g, Banlangen50g, Zicao50g, Tenghuang50g, Baizhi50g, Moyao50g, Kufan50g). Results: All of the patients were cure with 100% effective rate. Average period was 6 days, and all the patients were found no sequelae and recureence within two years^[1].

(2) 70 cases of facial herpes zoster patients were treated by Huzhangjiedu decoction(Huzhang15g, Banlangen20g, Huanglian4g, Huangqin10g, Shijieming30g, Chantui5g, Juhua10g, Danpi10g, Chishao10g, zicao10g, Baizhi10g, Gancao5g) with oral administration. Results showed that 29 cases were cure, 33 cases had significant effect and four cases' condition were impvoed, the overall response rate was 88.57% ^[2].

2. Cellulitis: Huzhang, Dahuan, Huangbai and Diyu were made into powder to treat 36 cases patients of Cellulitis. Results showed that 28 cases have significant effect and six cases had effect with the overall response rate were 94% ^[4].

3. Bedsore: Honghua lotion(Honghua 100g, Huzhang400g) combined with skin massage were used to prevent and treat 40 cases of bedsore. Results showed that 39 cases have effect with the overall response rate were 97.5% ^[4].

4. Chronic colonitis: Huzhang4g, prednisone 10mg and extract of scopolia were grind into powder and 60mL of salt water were added and mixed up, then used to treat patients of chronic colonitis with clysis before sleep and after cacation, with 15 days for one course of treatment. Results showed that 88 cases were cured (86.3%) and 14 cases' condition were improved (13.7%) within two course of treatment ^[5].

5. Acute joint sprain: Huzhang100g, Honghua20g, sumu50g were soak by 200mL ethanol with six months and filtrated, then the filtrate were used to embrocate on the sprain area of 57 cases of acute joint sprain with triple each day and last 5-7 days. Results showed that 52 cases were cured (91%) and five cases' condition were improved (9%)^[6].

6. Hepatic Fibrosis in early stage of chronic hepatitis B: 62 cases were treated with oral administration of Huzhang tablet. Results showed that 26 cases have significant effect and 27 cases have effect, the overall response rate were 93.55％ ^[7].

7. Radiodermatitis: 60 cases were embrocate Huzhang pigmentum on the affected area. Results showed that 40 cases were cured and 19 cases had effect after one course of treatment, the overall response rate were 98.33％^[8].

1.Stranguria

2.Amenorrhea

3.Internal abdominal mass like hard stone, with stabbing pain

4.Nameless disease due to qi rushing (subcutaneous sound, severe itching )

5.Consumptive thirst

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